Human papillomavirus DNA detection
What is this test?
This test detects DNA gene sequences of a type of virus called human papilloma virus (HPV) in cervical cells. It is used to detect potentially cancerous forms of this virus.
What are other names for this test?
- HPV DNA detection
What are related tests?
- Cervical cytology test
- Colposcopy of cervix
- Human papillomavirus genotyping
- Sampling of cervix for Papanicolaou smear
Why do I need this test?
Laboratory tests may be done for many reasons. Tests are performed for routine health screenings or if a disease or toxicity is suspected. Lab tests may be used to determine if a medical condition is improving or worsening. Lab tests may also be used to measure the success or failure of a medication or treatment plan. Lab tests may be ordered for professional or legal reasons. You may need this test if you have:
- Atypical squamous cells
- Screening for malignant neoplasm of cervix
When and how often should I have this test?
When and how often laboratory tests are done may depend on many factors. The timing of laboratory tests may rely on the results or completion of other tests, procedures, or treatments. Lab tests may be performed immediately in an emergency, or tests may be delayed as a condition is treated or monitored. A test may be suggested or become necessary when certain signs or symptoms appear.
Due to changes in the way your body naturally functions through the course of a day, lab tests may need to be performed at a certain time of day. If you have prepared for a test by changing your food or fluid intake, lab tests may be timed in accordance with those changes. Timing of tests may be based on increased and decreased levels of medications, drugs or other substances in the body.
The age or gender of the person being tested may affect when and how often a lab test is required. Chronic or progressive conditions may need ongoing monitoring through the use of lab tests. Conditions that worsen and improve may also need frequent monitoring. Certain tests may be repeated to obtain a series of results, or tests may need to be repeated to confirm or disprove results. Timing and frequency of lab tests may vary if they are performed for professional or legal reasons.
How should I get ready for the test?
Written consent will be required for endocervical sampling. Review the consent form with the healthcare worker and ask any questions that you have before signing the consent form.
Tell the healthcare worker if you have a medical condition or are using a medication or supplement that causes excessive bleeding. If possible, schedule the procedure one week after your menstrual period. Do not douche or have sexual intercourse for 24 hours before the procedure.
You may be asked to urinate prior to your endocervical sampling. This will make it easier for the healthcare worker to see your cervical canal during the procedure and may make the procedure more comfortable for you.
How is the test done?
For an endocervical sampling, you will be asked to lie on your back with your legs spread and feet placed in stirrups. A speculum will be inserted into your vagina. This tool is used to gently spread apart your vagina. A small brush is inserted into the endocervical canal and rotated. This is done to collect cells. Once a sufficient sample is collected, the brush is removed. The sample off the brush is then sent for testing.
How will the test feel?
The amount of discomfort you feel will depend on many factors, including your sensitivity to pain. Communicate how you are feeling with the person doing the procedure. Inform the person doing the procedure if you feel that you cannot continue with the procedure.
You may feel mild discomfort, cramping, or pain during an endocervical sampling.
What should I do after the test?
After endocervical sampling, you may experience some light spotting (mild, occasional bleeding from the vagina). Generally, there are no activity restrictions after this procedure.
What are the risks?
Endocervical cells: An endocervical cell sample is collected using a method similar to a Pap smear. This procedure may cause light bleeding from the vagina. If you have a medical condition, or are using a medication or supplement that causes excessive bleeding, you are at a higher risk of bleeding during or after this procedure. The person doing this procedure may need to perform it more than once. Talk to your healthcare worker if you have any concerns about the risks of this procedure.
What are normal results for this test?
Laboratory test results may vary depending on your age, gender, health history, the method used for the test, and many other factors. If your results are different from the results suggested below, this may not mean that you have a disease. Contact your healthcare worker if you have any questions. The following is considered to be a normal result for this test:
- Negative 
What follow up should I do after this test?
Ask your healthcare worker how you will be informed of the test results. You may be asked to call for results, schedule an appointment to discuss results, or notified of results by mail. Follow up care varies depending on many factors related to your test. Sometimes there is no follow up after you have been notified of test results. At other times follow up may be suggested or necessary. Some examples of follow up care include changes to medication or treatment plans, referral to a specialist, more or less frequent monitoring, and additional tests or procedures. Talk with your healthcare worker about any concerns or questions you have regarding follow up care or instructions.
Where can I get more information?
- Centers for Disease Control and Prevention (CDC) - http://www.cdc.gov/
- American Social Health Association (ASHA) - http://www.ashastd.org
- Division of STD Prevention, Centers for Disease Control and Prevention - http://www.cdc.gov/std
 Castle PE, Wheeler CM, Solomon D, et al: Interlaboratory reliability of Hybrid Capture 2. Am J Clin Pathol 2004; 122:238-245.
 Lorincz AT & Richart RM: Human papillomavirus DNA testing as an adjunct to cytology in cervical screening programs. Arch Pathol Lab Med 2003; 127:959-968.
 National Comprehensive Cancer Network: Cervical cancer V.1.2008. National Comprehensive Cancer Network. Jenkintown, PA. 2007. Available from URL: http://www.nccn.org/professionals/physician_gls/PDF/cervical.pdf. As accessed March 11, 2008.
Last Updated: 12/4/2015